Analysis of 30+ million partitions on UltraPCR is simple. UltraPCR leverages a proprietary emulsion chemistry that is thermally stable and optically clear such that we can directly image the PCR tube using 3D imaging.
UltraPCR
Others
Single-tube workflow from partitioning to analysis eliminates risks of cross-sample contamination.
UltraPCR Imager can scan 30+ million partitions in under a minute per channel.
Immobilized emulsions combined with serial imaging creates "optical signature" profiles of each positive partition, enabling more fluorescent dyes to be used on the UltraPCR system.
Efficient partitioning without microfluidics with no dead volume
Centrifugation-based partitioning using a standard benchtop centrifuge
30+ million partitions per sample directly placed into PCR tubes, 1000X+ more partitions than conventional dPCR
Industry-leading 50 uL PCR reactions with no dead volume
Counting DNA the way it should be - 1 molecule at a time.
Singlet partitions in UltraPCR
Mixed molecule partitions in dPCR
30+million partitions enables >1 million molecules, each in its own “singlet” partition
Count with simplicity and confidence - 1 positive singlet partition equals 1 DNA molecule
Eliminate the need of Poisson statistics arising from limited partitions in legacy dPCR systems
True single molecule counting over an unmatched 6-log dynamic range, achieving the true concept of digital PCR
Clarity in 3D provides direct peek of singlet partitions directly from PCR tubes.
Ultra-dPCR
Other dPCR
Proprietary chemistry enables formation of thermally stable and optically clear partitions for rapid, high-resolution 3D scanning in the same tube
Positive partitions are counted directly from within the PCR tube, providing a streamlined, single-tube workflow and eliminating risks of cross-sample contaminations
UltraPCR platform uses lightsheet imaging to provide high resolution 3D microscopy directly in PCR tubes with clear partitions
UltraClear 3D can scan 34 million partitions in under a minute per channel
Bringing multiplexing for everyday use.
Novel multiplexing alternative using a standard pair of PCR primers without the need of expensive hydrolysis probes
Rapid, low cost, multiplexed panel design (up to 4 with standard product offering)
Comparable counting performance as hydrolysis probes
Unprecedented level of multiplexing unlocked in UltraPCR.