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Massively parallel partitioning using simple spin columns
30+ million partitions per sample are generated using a standard centrifuge, generating >1500X more partitions than conventional dPCR
Advantages:
Simple design eliminates the need for complex microfluidics, so this process can be performed in most molecular biology labs.
Spin column format enables industry-leading PCR volume of 50uL, suitable for diluted samples such as cell free DNA (cfDNA) in search for rare molecules.
High centrifugal force processes the entire sample with no dead volume, adding to the sensitivity of UltraPCR for rare molecule dection
UltraPartitioning™
Efficient partitioning without microfluidics with no dead volume
Centrifugation-based partitioning using a standard benchtop centrifuge
30+ million partitions per sample directly placed into PCR tubes, 1000X+ more partitions than conventional dPCR
Industry-leading 50 uL PCR reactions with no dead volume
Singlet Counting™
Counting DNA the way it should be - 1 molecule at a time.
Singlet partitions in UltraPCR
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Mixed molecule partitions in dPCR
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30+million partitions enables >1 million molecules, each in its own “singlet” partition
Count with simplicity and confidence - 1 positive singlet partition equals 1 DNA molecule
Eliminate the need of Poisson statistics arising from limited partitions in legacy dPCR systems
True single molecule counting over an unmatched 6-log dynamic range, achieving the true concept of digital PCR
UltraClear 3D™
Clarity in 3D provides direct peek of singlet partitions directly from PCR tubes.
Ultra-dPCR
Other dPCR
Proprietary chemistry enables formation of thermally stable and optically clear partitions for rapid, high-resolution 3D scanning in the same tube
Positive partitions are counted directly from within the PCR tube, providing a streamlined, single-tube workflow and eliminating risks of cross-sample contaminations
UltraPCR platform uses lightsheet imaging to provide high resolution 3D microscopy directly in PCR tubes with clear partitions
UltraClear 3D can scan 34 million partitions in under a minute per channel
Universal Multiplex™
Bringing multiplexing for everyday use.
Novel multiplexing alternative using a standard pair of PCR primers without the need of expensive hydrolysis probes
Rapid, low cost, multiplexed panel design (up to 4 with standard product offering)
Comparable counting performance as hydrolysis probes
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